Zhang Lab

Location and Contact Information

630 West 168th Street
PS10-401
New York, NY 10032
United States

Principal Investigators

The Zhang laboratory in the Department of Medicine at Columbia University Medical Center is interested in the role of macrophages in cardiometabolic diseases. The main areas of study in the Zhang laboratory include: (1) Functional validation of candidate genes and genetic variants identified in genome-wide association studies of cardiometabolic traits; (2) Unbiased CRISPR screen in primary macrophages to discover novel regulators of macrophage efferocytosis; (3) The role of human macrophage long noncoding RNAs in macrophage biology.

Dr. Zhang is a member of the Arteriosclerosis Thrombosis and Vascular Biology (ATVB) Women's Leadership Committee (WLC). It is her mission and passion to support the career advancement of the next generation of translational scientists. Potential students, postdoctoral trainees, and visiting scientists are encouraged to contact Dr. Zhang at hz2418@cumc.columbia.edu for opportunities to work in the laboratory.

Lab Members

  • Hanrui Zhang MB PhD

    Assistant Professor of Medicine

    Dr. Hanrui Zhang is a macrophage biologist and cardiovascular physiologist. Her research addresses the mechanisms of cardiometabolic diseases with a specific focus on the multi-dimensional role of macrophages in lipid metabolism and innate immunity using functional genomic approaches, RNA-sequencing, human induced pluripotent stem cells (iPSCs), CRISPR/Cas9 gene editing, murine models, and immunological and molecular biological methods. The long-term goal of the Zhang laboratory is to elucidate the mechanisms and therapeutic implications of macrophage heterogeneity and plasticity in cardiometabolic diseases.

    Dr. Zhang is a member of the Arteriosclerosis Thrombosis and Vascular Biology (ATVB) Women's Leadership Committee (WLC). It is her mission and passion to support the career advancement of the next generation of translational scientists. Potential students, postdoctoral trainees and visiting scientists are encouraged to contact Dr. Zhang at hz2418@cumc.columbia.edu for opportunities to work in the laboratory.

    Education & Training

    MB, 2003, Beijing University of Chinese Medicine, Beijing, China

    MS, 2006, Beijing University of Chinese Medicine, Beijing, China

    PhD, 2011, University of Missouri, Columbia, MO

  • Jianting Shi MS

    Research Specialist

    My work focuses on exploring the functionality of myeloid cells and their roles in cardiometabolic diseases using genome-wide CRISPR screen and next-gen sequencing technologies.

    Education and Training
    BE, 2010, Guangdong University of Technology, Guangzhou, China
    MS, 2014, UMDNJ (now Rowan University), Stratford, NJ

     

Select Publications

  • Zhang H, Xue C, Shah R, Bermingham K, Hinkle CC, Li W, Rodrigues A, Tabita-Martinez J, Millar JS, Cuchel M, Pashos EE, Liu Y, Yan R, Yang W, Gosai SJ, VanDorn D, Chou ST, Gregory BD, Morrisey EE, Li M, Rader DJ, Reilly MP. Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling Mendelian disease. Circulation research. 2015 Jun 19;117(1):17-28. PMCID: PMC4565503

  • Zhang H*, Shi J, Hachet MA, Xue C, Bauer RC, Jiang H, Li W, Tohyama J, Millar J, Billheimer J, et al. CRISPR/Cas9-Mediated Gene Editing in Human iPSC-Derived Macrophage Reveals Lysosomal Acid Lipase Function in Human Macrophages. Arterioscler Thromb Vasc Biol. 2017 Sep 7. pii: ATVBAHA.117.310023. PMCID: PMC5659288

  • Zhang H, Xue C, Wang Y, Shi J, Zhang X, Li W, Nunez S, Foulkes AS, Lin J, Hinkle CC, Yang W, Morrisey EE, Rader DJ, Li M, and Reilly MP*. Deep RNA-sequencing uncovers a repertoire of human macrophage lincRNAs that is modulated by macrophage activation and associates with cardiometabolic diseases. J Am Heart Assoc. 2017 Nov 13;6(11). pii: e007431. PMCID: PMC5721798.

  • Zhang H*, Hinkle CC, O'Neill SM, Shi J, Caughey J, Lynch E, Lynch G, Gerelus M, Tsai ASD, Shah R, Ferguson JF, Ahima RS, Reilly MP*. Synergistic Modulation of Inflammatory but not Metabolic Effects of High-Fat Feeding by CCR2 and CX3CR1. Obesity (Silver Spring). 2017 Aug;25(8):1410-1420. PMCID: PMC5610963.

  • Zhang H*, Reilly MP. Human iPSC-derived macrophages for unraveling human macrophage biology. Arterioscler Thromb Vasc Biol. 2017;37:2000-2006. PMCID: PMC5687272. Review.

  • Zhang H*. Lysosomal acid lipase and lipid metabolism: new mechanisms, new questions, and new therapies. Curr Opin Lipidol. 2018 Jun;29(3):218-223. PMID: 29547398. PMCID Pending. Review.