Hanrui Zhang, PhD

Profile Headshot

Overview

Academic Appointments

  • Assistant Professor of Medical Sciences (in Medicine)

Dr. Hanrui Zhang is a macrophage biologist and cardiovascular physiologist. She completed her PhD training in 2011 at the University of Missouri. Her graduate work centered on the crosstalk between immune cells and blood vessels in diabetes-associated vascular diseases. Her postdoctoral research at the University of Pennsylvania continued to address the mechanisms of cardiometabolic diseases focusing on macrophage lipid metabolism and inflammation using gene targeting in human induced pluripotent stem cell (iPSC) with differentiation to macrophages.

The Zhang laboratory (https://hanruizhang.github.io/zhanglab/) in the Department of Medicine – Cardiology at Columbia University Irving Medical Center seeks to understand the dynamic role of macrophages in cardiometabolic diseases with the aim of finding novel mechanisms and new treatments. The laboratory applies technologies for high-throughput functional genomics, human iPSC and CRISPR gene editing, transgenic mouse models, human genetics, and a variety of cell and molecular techniques.

Gender

  • Female

Credentials & Experience

Education & Training

  • PhD, 2011 Pharmacology, University of Missouri - Columbia

Research

The goal of the Zhang laboratory is to elucidate the mechanisms and therapeutic implications of macrophage heterogeneity and plasticity in cardiometabolic diseases.

The Zhang laboratory in the Department of Medicine – Cardiology at Columbia University Irving Medical Center seeks to understand the dynamic role of macrophages in cardiometabolic diseases with the aim of finding novel mechanisms and new treatments. The laboratory applies technologies for high-throughput functional genomics, human iPSC and CRISPR gene editing, transgenic mouse models, human genetics, and a variety of cell and molecular techniques.

The main areas of study in the laboratory include: (1) Functional genomics and mechanistic studies of candidate genes and genetic variants inspired by human genome-wide association studies of cardiometabolic traits; (2) Unbiased CRISPR screen in primary macrophages to discover novel regulators and pathways of macrophage efferocytosis and investigate their roles in atherosclerosis; (3) The role of human macrophage long noncoding RNAs in macrophage biology.

Grants

ELUCIDATION OF TISSUE-SPECIFIC TRANSCRIPTOMIC PROFILES IN CARDIO-METABOLIC DISEASE (Federal Gov)

Jul 1 2018 - Jul 31 2023

MACROPHAGE-SPECIFIC FUNCTION OF GWAS CAD-ASSOCIATED LIPA ALLELES IN ATHEROSCLEROSIS (Federal Gov)

Jul 1 2018 - Aug 31 2021

HUMAN LINCRNAS IN MACROPHAGE BIOLOGY AND RELATED CARDIOMETABOLIC DISEASES (Federal Gov)

Aug 1 2017 - Jul 31 2021

MACROPHAGE-SPECIFIC FUNCTION OF GWAS CAD-ASSOCIATED LIPA ALLELES IN ATHEROSCLEROSIS (Federal Gov)

Jul 1 2018 - Jun 30 2021

IMPACT OF CAD-ASSOCIATED VARIANTS IN LIPA ON ATHEROGENTIC PHENOTYPES IN HUMAN MACROPHAGES (Private)

Feb 1 2016 - Jun 30 2017

Selected Publications

  • Li F, Zhang H*. Lysosomal Acid Lipase in Lipid Metabolism and Beyond. Arterioscler Thromb Vasc Biol. 2019 Mar 14:ATVBAHA119312136.
  • Shi J, Xue C, Liu W, Zhang H*. Differentiation of Human Induced Pluripotent Stem Cells to Macrophages For Disease Modeling and Functional Genomics. Current Protocols in Stem Cell Biology. 2019 Feb;48(1):e74.
  • Westerterp M, Fotakis P, Ouimet M, Bochem AE, Zhang H, Molusky MM, Wang W, Abramowicz S, la Bastide-van Gemert S, Wang N, Welch CL, Reilly MP, Stroes ES, Moore KJ, Tall AR. Cholesterol Efflux Pathways Suppress Inflammasome Activation, NETosis and Atherogenesis. Circulation. 2018 Aug 28;138(9):898-912.
  • Zhang H*, Reilly M*. Who done it? Macrophage Mayhem in Atherosclerosis. Circ Res. 2018 Oct 26;123(10):1106-1108.
  • Zhang H*, Xue C, Wang Y, Shi J, Zhang X, Li W, Nunez S, Foulkes AS, Lin J, Hinkle CC, Yang W, Morrisey EE, Rader DJ, Li M, and Reilly MP*. Deep RNA-sequencing uncovers a repertoire of human macrophage lincRNAs that is modulated by macrophage activation and associates with cardiometabolic diseases. J Am Heart Assoc. 2017 Nov 13;6(11).
  • Zhang H*, Shi J, Hachet MA, Xue C, Bauer RC, Jiang H, Li W, Tohyama J, Millar J, Billheimer J, Phillips MC, Razani B, Rader DJ, Reilly MP. CRISPR/Cas9-Mediated Gene Editing in Human iPSC-Derived Macrophage Reveals Lysosomal Acid Lipase Function in Human Macrophages. Arterioscler Thromb Vasc Biol. 2017 Nov;37(11):2156-2160.
  • Nurnberg ST, Zhang H, Hand NJ, Bauer RC, Saleheen D, Reilly MP and Rader DJ. From Loci to Biology: Functional Genomics of Genome-Wide Association for Coronary Disease. Circulation Research. 2016;118:586-606.
  • Zhang H, Xue C, Shah R, Bermingham K, Hinkle CC, Li W, Rodrigues A, Tabita-Martinez J, Millar JS, Cuchel M, Pashos EE, Liu Y, Yan R, Yang W, Gosai SJ, VanDorn D, Chou ST, Gregory BD, Morrisey EE, Li M, Rader DJ, Reilly MP. Functional analysis and transcriptomic profiling of iPSC-derived macrophages and their application in modeling Mendelian disease. Circulation Research. 2015 Jun 19;117(1):17-28.